PhD course on DNA and mRNA quantification in Molecular Microbial Ecology - 08.03.2010

Time: 12- 16 April, 2010

 

Location: Øster Voldgade 10, 1350 - Copenhagen, Denmark

 

Level: PhD

 

Description: Assessment of presence and activity of microorganisms in natural waters, fecal samples and soil has during the last years been revolutionized by introduction of highly reliable methods to extract both DNA (for presence) and mRNA (for activity) from microorganisms present in the environmental sample.

The newest technology within extraction of mRNA directly from water and soil samples is based on snap shot freezing and beadbeating in the presence of phenol and chloroform. This technology has been proved to work in quantification of gene expression of functional genes in soil samples, and the activity has been shown to be linked to the activity of the organisms.

In this course we will quantify mRNA and DNA of the invA gene in Salmonella directly in soil and water.

The potential of using quantitative PCR for tracking pathogens in the environment based on direct extracted DNA from water-, fecaland soil samples will be an other element of the course—targeting different types of pathogens and indication organisms including E.coli and Bacteroides sp.

 

Organizer: GEUS - Geological Survey of Denmark and Greenland

 

Contact:

 

Fee: The course will have a fee of 3.000

DKK to cover lab expenses, lunch and

social events

 

Application:Informal enquiries as well as

registration should be send to course

responsible Carsten Suhr Jacobsen

 

For further information read the flyer here